Intermediate

Part:BBa_K1372000:Design

Designed by: Fabio Maschi, Sylvie Lautru   Group: iGEM14_Paris_Saclay   (2014-10-02)


salicylate inducible suppressing system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312


Design Notes

assembly of the BBa_J61051 biobrick (PSal/NahR biosensor) with the BBa_K228001 biobrick (SupD-tRNA)


Source

biobricks BBa_J61051 and BBa_K228001

References